To investigate the colonization of endophytic nitrogen-fixing bacterium Klebsiella variicola in Penniseum sinense, pET28a and the upgraded green fluorescent protein (EGFP) were utilized as essential components to develop a recombinant articulation vector pET28a-Lac-EGFP with the expansion of starter Lac in pUC18 vector utilizing enzymatic processing and grafting strategies. The built recombinant vector was changed into… Continue reading