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Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA)

Posted on November 26, 2021 by Caroline

Product overview

The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) gear (ab213839) is designed for the quantitative measurement of Human TNFRSF13C in cell customized supernatants, cell lysates, serum and plasma (heparin, EDTA).

The ELISA gear relies on commonplace sandwich enzyme-linked immune-sorbent assay expertise. A monoclonal antibody from mouse specific for BAFF-R has been pre-coated onto 96-well plates. Requirements (Expression system for common: NSO; Immunogen sequence: S7-A71) and try samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF-R is added subsequently after which adopted by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Subtle is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualise HRP enzymatic response. TMB is catalyzed by HRP to supply a blue shade product that turned yellow after along with acidic cease reply. The density of yellow is proportional to the Human BAFF-R quantity of pattern captured in plate.

Key decisions and particulars

  • Sensitivity: 10 pg/ml
  • Differ: 78 pg/ml – 5000 pg/ml
  • Pattern kind: Cell customized supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay kind: Sandwich (quantitative)
  • Reacts with: Human
  • Product set up

    Human BAFF-R ELISA Instruments
  • Detection method

    Colorimetric
bio human baff r
bio human baff r
  • Precision

    Intra-assay
    Pattern n Point out SD CV%
    1 16 147pg/ml 10.29 = 7%
    2 16 683pg/ml 49.85 = 7.3%
    3 16 2750pg/ml 206.25 = 7.5%
    Inter-assay
    Pattern n Point out SD CV%
    1 24 156pg/ml 13.88 = 8.9%
    2 24 700pg/ml 62.3 = 8.9%
    3 24 2531pg/ml 227.05 = 8.9%
  • Pattern kind

    Cell customized supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma
  • Assay kind

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Differ

    78 pg/ml – 5000 pg/ml
  • Assay time

    3h 30m
  • Assay dimension

    Loads of steps commonplace assay
  • Species reactivity

    Reacts with: Human

 

  • Notes

    BAFF-R (moreover generally known as Tumor necrosis problem receptor superfamily member 13C or B-cell-activating problem receptor)  is a protein that in people is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating problem (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell inhabitants. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich area. It’s thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.

  • Platform

    Pre-coated microplate (12 x Eight appropriately strips)

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  • EUR 361.02
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Histone Acetyl H4K5ac Peptide

R-1014 EpiGentek
  • EUR 267.06
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  • 200 µl
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Histone Acetyl H4K8ac Peptide

R-1015 EpiGentek
  • EUR 267.06
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Histone Acetyl H4K12ac Peptide

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  • EUR 267.06
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  • 200 µl
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Histone Acetyl H4K16ac Peptide

R-1017 EpiGentek
  • EUR 267.06
  • EUR 78.10
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  • 200 µl
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Histone H3K4me1 Monomethyl Peptide

R-1018 EpiGentek
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
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Histone H3K4me1 Monomethyl Peptide, Biotinylated

R-1019 EpiGentek
  • EUR 361.02
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  • 200 µl
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Histone H3K4me2 Dimethyl Peptide

R-1020 EpiGentek
  • EUR 267.06
  • EUR 78.10
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Histone H3K4me2 Dimethyl Peptide, Biotinylated

R-1021 EpiGentek
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
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Histone H3K4me3 Trimethyl Peptide

R-1022 EpiGentek
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
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Histone H3K4me3 Trimethyl Peptide, Biotinylated

R-1023 EpiGentek
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
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Histone H3K9me1 Monomethyl Peptide

R-1024 EpiGentek
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
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Histone H3K9me1 Monomethyl Peptide, Biotinylated

R-1025 EpiGentek
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K9me2 Dimethyl Peptide

R-1026 EpiGentek
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K9me2 Dimethyl Peptide, Biotinylated

R-1027 EpiGentek
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K9me3 Trimethyl Peptide

R-1028 EpiGentek
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K9me3 Trimethyl Peptide, Biotinylated

R-1029 EpiGentek
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K27me1 Monomethyl Peptide

R-1030 EpiGentek
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K27me1 Monomethyl Peptide, Biotinylated

R-1031 EpiGentek
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K27me2 Dimethyl Peptide

R-1032 EpiGentek
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K27me2 Dimethyl Peptide, Biotinylated

R-1033 EpiGentek
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K27me3 Trimethyl Peptide

R-1034 EpiGentek
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
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Histone H3K27me3 Trimethyl Peptide, Biotinylated

R-1035 EpiGentek
  • EUR 361.02
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